HIV Eradication in vitro PDF Print E-mail
Written by Alain Lafeuillade   
Wednesday, 17 November 2010 15:44

Specific eradication of HIV-1 from infected cultured cells

Levin A, Hayouda Z, Friedler A, Loyter A. AIDS Res Ther 2010; 7: 31

A
n orginal study performed in vitro suggests that encouraging proviral integration in cells would be a way to obtain eradication... Previous findings have shown that multi-integration of viral DNA in cells may lead to host genome instability. This work follows previous reports from the same group showing that the integrase activity is restricted by an early expressed viral Rev protein and the isolation of integrase-related peptides that stimulate genome integration.

T
hese peptides, called INS and INrs, were added together with an HIV protease inhibitor (RO 31-8959, which is in fact saquinavir) to cell cultures. The combination of these 3 components was named the "Mix".

An increase in virus production and in viral cDNA integration was observed during the first 2-4 days. Then, a drastic reduction in virus production and cDNA integration was observed, and 40% of the cells died by the 8th day, of apoptosis. For the authors, there is proof that the infection process is terminated and HIC cleared from culture.

We think that there results should be taken with caution as in vitro results have often been far from the patient reality...


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Key words: culture, cure, eradication, hiv, in vitro, peptide, proviral reservoir
Last Updated on Friday, 19 November 2010 17:00
 

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