Failure of Autologous HSCT In Rhesus Macaques PDF Print E-mail
Written by Alain Lafeuillade   
Wednesday, 01 October 2014 07:37

Failure of Autologous Hematopoietic Stem Cell Transplant In Rhesus Macaques to Control the Reservoir

rhesus-macaqueIn a recent paper published in PLOS Pathogens, 3 SHIV-infected Rhesus Macaques received HSCT collected prior to infection.

Despite a drmatic decrease of SHIV reservoir with the pre-transplant conditioning, including Total Body Irradiation (TBI), plasma viremia relapsed and 2 and SHIV DNA was still detected in the third, after ART was stopped.

 

 

The first author of this paper is M. Mavignier from Emory Vaccine Center.

 

Three RMs were taken as controls and 3 as experiment. All were infected with SHIV and all started ART 4 weeks after infection: Tenofovir/FTC/Efavirenz/Raltegravir.

 

In the 3 experiments; collection of stem cells was performed before infection. After 5-8 weeks on ART, they received myeloablative TI as pre-transplant conditioning. This resulted in the depletion of 94.2-99.2% of circulating CD4 T cells.

 

ART was interrupted after stem cell engraftment (78-128 days post-initiation).

 

Rapid rebound of plasma viremia was observed in 2 RM, while the third has to be euthanized due to renal failure and showed SHIV DNA in CD4 cells, splenn and lymph nodes at autopsy.

 

Mavignier M et al. PLOS Pathogens 2014; 10 (9): e1004406




Key words: HIV DNA, HIV Persistance, HIV reservoirs, Rhesus Macaques, SHIV, SIV
Last Updated on Wednesday, 01 October 2014 08:15
 

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