HIV Reservoir Larger than Previously Estimated? PDF Print E-mail
Written by Roger Leroy   
Tuesday, 05 March 2013 16:07

HIV Reservoir Larger than Previously Estimated?

measureTerrific studies from Robert Siliciano laboratory tend to show that the resting T cell reservoir of HIV is larger than previously estimated.

The authors have analysed HIV DNA in cells after outgrowth assays and genetically characterized proviruses that were not reactivated after stimulation.

These data have been presented at the 2013 CROI by both Professor Siliciano in a plenary lecture and Ya-Chin Ho from his lab.

 

Using the viral outgrowth assay, which is the reference test to measure the latent HIV reservoir, they found that around 1/106 cells induced proviruses while 300/106 cells did not.

These non-induced proviruses were then characterized.

The authors show that 87.8% (187/213) of non-induced proviruses are defective, while 12.2% have intact proviral genomes, are replication competent, have normal LTR functions and replication fitness (figure).

 

 

Defective-proviruses

 

 

Consequently, intact non-induced proviruses are 48 fold more frequent than induced proviruses. This could mean that the latent reservoir for HIV may have been underestimated and would be around 50 fold larger than previously thought.



 


Key words: HIV curative strategies, HIV cure, HIV persistence, HIV reservoirs
Last Updated on Wednesday, 06 March 2013 13:11
 

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